They included Catholic groups and other backers of traditional family rights.
President Francois Hollande has promised to change French law so that gay and lesbian couples can marry.
Despite opposition from more than 1,000 mayors and the Catholic Church, his socialist government approved a bill on the issue earlier this month which will be debated by parliament in January.
France already allows civil unions between same-sex couples, but extending their rights was a campaign pledge of Mr Hollande before he was elected in May.
Pink balloons
Protesters in Paris wore pink T-shirts and scarves and carried pink balloons emblazoned with images of a man and woman holding two children's hands.
Saturday also saw counter-rallies in support of same-sex marriage.
The issue is one of the most divisive Mr Hollande has faced, correspondents say. The head of the French Council of Catholic Bishops, Cardinal Andre Vingt-Trois, recently described gay marriage as "the ultimate deceit".
At present only married couples - not civil union partners - can adopt in France.
A number of European nations, including Sweden and the UK, already allow gay adoption.