Emergency crews attended Pollokshaws Parish Church in Glasgow after they received reports of the blaze at around 2.44am.
The building, famous for its Georgian villa style and dating back to 1843, appears gutted in pictures and social media.
With three appliances, the fire and rescue service remains on the scene. It is currently unclear what started the fire of if there are any injuries.
In a statement, Glasgow City Council said: "Shawbridge Street between both terminations of Shawholm Crescent is currently closed to traffic due to a fire.
"Emergency services are on location.
"Road users are advised to use alternative routes and delays are to expected."
Locals have shared their sadness, with one writing online: "Absolutely devastated about Pollokshaws Parish Church been burnt down that was the family church away back as far as my nanna and grandad."
A Scottish Fire & Rescue Service spokesperson said: "We received a call at 2:44am on Sunday to reports of a building on fire on Shawbridge Street in Glasgow.
“Crews remain in attendance.”